A Colorimetric Method for the Determination of Serum Arginase Activity

Author:

Loeb Walter F1,Stuhlman Robert A1

Affiliation:

1. College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210

Abstract

Abstract A detailed description of a method for the determination of serum arginase activity is presented. Arginase activity is demonstrated by the decrease in arginine concentration in a reaction mixture, as determined by a modification of the method of Sakaguchi. The reaction is linear until inhibited by the increasing concentration of ornithine. The cellular distribution of arginase is reviewed to determine its role in diagnosis and in the evaluation of biologic systems.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

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