Affiliation:
1. Biochemistry Laboratory, U. S. Army Research Institute of Environmental Medi cine, Natick, Mass. 01762
Abstract
Abstract
A spectrophotometric method for the direct determination of microgram quantities of calcium in serum is presented. In this procedure, 0.02 ml. of serum is interacted with buffered glyoxal bis (2-hydroxyanil) solution (GBHA) at pH 12.6. The resultant red calcium-GBHA complex is diluted in methanol-acetone-water solution, and the absorbance determined at 520 mµ. The effects of pH, temperature, GBHA concentration, and alcohol-water ratio were evaluated, as was interference by serum constituents. The method is simple and precise, and the analytical results compare well with the chloranilate procedure for serum calcium.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
19 articles.
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