Affiliation:
1. Department of Pathology, Veterans Administration Hospital, Wilmington, Dela
Abstract
Abstract
A rapid, simple, highly accurate procedure for the determination of hemoglobin iron is described. The use of a sodium hypochlorite solution achieves a smoother, faster, cleaner digestion of hemoglobin and release of the iron than the use of concentrated sulfuric acid and persulfate.
The accuracy of the method is established by recovery experiments showing that a linear relation exists between the volume of a given hemoglobin solution analyzed and the total amount of iron released, as well as by the demonstration that a constant fixed ratio exists between hemoglobin iron and hemoglobin (as cyanmethemoglobin).
The new method is easier to perform than the time-honored procedure of Wong.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
12 articles.
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