Improved Method for Measurement of delta-Aminolevulinic Acid Dehydratase Activity of Human Erythrocytes-Reference-Cited by-同舟云学术

Improved Method for Measurement of delta-Aminolevulinic Acid Dehydratase Activity of Human Erythrocytes

Published:1971-10-01 Issue:10 Volume:17 Page:1038-1041
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Burch Helen B¹,Siegel Abraham L¹

Affiliation:

1. Trace Metals Laboratory, Veterans Administration Hospital, Birmingham, Ala., and the Department of Clinical Pathology, University of Alabama School of Medicine, Birmingham, Ala. 35233

Abstract

Abstract We have evaluated and modified a current method for estimating δ-aminolevulinic acid dehydratase (5-aminolaevulinate hydro-lyase, EC 4.2.1.24) activity in blood, so as to adequately control the pH of the enzyme-substrate solution at the optimum throughout the incubation period. This has improved the sensitivity and reliability of the assay. Also, N-ethylmaleimide has been substituted for a potentially hazardous mercury salt, used to remove sulfhydryl groups before color development with a modified Ehrlich’s reagent.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/17/10/1038/32823881/clinchem1038.pdf

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