Determination of Free Fatty Acids in Plasma by a Colorimetric Procedure: An Appraisal of the Method and Comparison with Other Technics-Reference-Cited by-同舟云学术

Determination of Free Fatty Acids in Plasma by a Colorimetric Procedure: An Appraisal of the Method and Comparison with Other Technics

Published:1965-08-01 Issue:8 Volume:11 Page:741-747
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Anstall Harold B¹,Trujillo Jose M¹

Affiliation:

1. Section of Research Clinical Pathology, Department of Pathology, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Tex

Abstract

Abstract A microcolorimetric procedure for measurement of long-chain fatty acids in pure solution was adapted to eliminate phospholipid interference and used to determine free fatty acids (FFA) in human plasma and serum. The lipids were extracted from plasma into chloroform; the phospholipids precipitated with acetone and MgCl2 in water bath; and the FFA concentration in the supernatant determined by formation of copper soaps. The copper was assayed colorimetrically. The method was used to establish the range of normal values for plasma FFA in human subjects.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/11/8/741/32818012/clinchem0741.pdf

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