Development of an agarose gel electrophoresis technique for determining alpha-amylase isoenzymes.

Author:

Royse V L,Jensen D M

Abstract

Abstract Evaluation of alpha-amylase isoenzymes as a clinical diagnostic aid by previous methodologies has been either too insensitive or too cumbersome for routine clinical laboratory use. With use of an agarose gel electrophoresis system (Worthington Diagnostic Systems, Inc.) serum amylase activity can easily be resolved into at least nine isoenzymes, the resolution being comparable with that of isoelectric focusing. Samples can be analyzed in less than one working day, with use of conventional reagents.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Cited by 15 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Nanocellulose for gel electrophoresis;Journal of Colloid and Interface Science;2019-03

2. In situ detection of starch-hydrolyzing microorganisms in activated sludge;FEMS Microbiology Ecology;2008-11

3. Glycosylated salivary α-amylases are capable of maltotriose hydrolysis and glucose formation;Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology;2000-08

4. Determination of total and pancreatic α-amylase in human serum with 2-chloro-4-nitrophenyl-α-d-maltotrioside as substrate;Clinica Chimica Acta;1997-03

5. Hydrolases acting on glycosidic bonds: chromatographic and electrophoretic separations;Journal of Chromatography B: Biomedical Sciences and Applications;1996-09

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3