Calculation of radioimmunoassay standard curves.

Author:

Naus A J,Kuppens P S,Borst A

Abstract

Abstract A method for calculating radioimmunoassay standard curves, based on the theory of Ekins et al., is described. Because a four-parameter model is used, nonlinear standard curves are the result. The calibration curve is fitted to the measured standard points by means of a weighted least-squares method. The program based on this model can be easily processed on a desk-top calculator. For all 250 runs of six different assays, very good standard curves could be obtained. The mean deviation between the concentrations of the standard points and the corresponding calculated values was about 6%. In 26% of the cases it could be shown that the model we describe gave significantly better results than did two simpler ones.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Cited by 15 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Radioimmunoassay;Wiley StatsRef: Statistics Reference Online;2014-09-29

2. Radioimmunoassay;Encyclopedia of Biostatistics;2005-07-15

3. Modified and improved anti-acetylcholine receptor (AChR) antibody assay: comparison of analytical and clinical performance with conventional anti-AChR antibody assay;Clinical Chemistry;1997-05-01

4. La place actuelle de l'immunoanalyse en toxicologie clinique;Immuno-analyse & Biologie Spécialisée;1996-01

5. Transforming Signals into Quantitative Values and Mathematical Treatment of Data;Scandinavian Journal of Clinical and Laboratory Investigation;1991-01

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