Abstract
Abstract
We describe an automated method for measuring the elastase-inhibitory capacity of plasma. We use a GEMSAEC centrifugal analyzer and succinyltrialanine-p-nitroanilide as an elastase substrate. The method is fast (80--100 samples/h) and accurate (CV = 3.6%). The detection limits are from 20 to 300% of the normal value. The elastase-inhibitory capacity is well correlated with the trypsin-inhibitory capacity and with the immunochemically determined concentration of alpha 1-proteinase inhibitor (alpha 1-antitrypsin). Because elastase is not inhibited by other plasma inhibitors, this method can be used as an enzymatic technique for measuring the concentration of plasma alpha 1-proteinase inhibitor. In an apparently normal population, this concentration was 40 +/- 11 mumol/L.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
22 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献