Affiliation:
1. Surgical Research Laboratories, Department of Surgery, St. Vincent's Hospital of the City of New York, New York 11, N. Y., and New York University School of Medicine
Abstract
Abstract
An accurate, reproducible colorimetric method for determining minute amounts of alkylating agents in biologic fluids has been developed. Utilizing this method, we have been able to detect less than 0.1 µg. of nitrogen mustard (HN2), or Thio-TEPA in 1 ml. of plasma, lymph, urine, or tissue homogenate. The biologic fluid was deproteinized with zinc sulfate, barium hydroxide, and methanol; the supernatant was used to alkylate γ-(4-nitrobenzyl) pyridine (NBP) under optimum conditions. The color of the quaternary pyridinium NBP derivative was extracted into ethyl acetate and read on a Beckman DU spectrophotometer utilizing microcells.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
19 articles.
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