Affiliation:
1. Sharon Research Institute and the Sharon Hospital, Sharon, Conn. 06069
Abstract
Abstract
Accuracy of the automated fluorometric measurement of triglyceride by the technique of Kessler and Lederer is limited by the instability of the baseline. Concentration of the alcoholic KOH reagent was modified, and the pump tube manifold altered to provide on-stream mixing of the KOH reagent, which overcame this difficulty. A relatively inexpensive fluorometer with the solid-state photomultiplier microphotometer, modified to provide dual-scale sensitivity, was used to increase precision of measurements. Analytical results for 30 serum samples, taken from three pools of frozen serum, show the accuracy of the measurements to be ±8 mg/100 ml and the standard deviation ±9 mg/100 ml for a single determination.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
75 articles.
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