A Simple Method for the Quantitative Determination of N-Acetyl-p-Aminophenol (APAP) in Urine-Reference-Cited by-同舟云学术

A Simple Method for the Quantitative Determination of N-Acetyl-p-Aminophenol (APAP) in Urine

Published:1965-12-01 Issue:12 Volume:11 Page:1064-1067
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Welch Richard M¹,Conney A H¹

Affiliation:

1. Wellcome Research Laboratories, Burroughs Wellcome & Co. (U.S.A.) Inc., Tuckahoe, N. Y., and the Department of Pharmacology, Albert Einstein College of Medicine, Bronx, N.Y

Abstract

Abstract A simple method is described for the rapid quantitative analysis of N-acetyl-p-amino-phenol (APAP) in urine. APAP and its conjugates present in the urine following the ingestion of acetophenetidin, APAP or acetanilid, are hydrolyzed with acid to p-aminophenol. This compound is coupled with phenol in the presence of hypobromite to form an indophenol dye whose concentration is determined spectrophotometrically. Application of this method to a study of 3 human subjects treated with acetophenetidin is described.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/11/12/1064/32818340/clinchem1064.pdf

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