Separation and Quantitation of Coproporphyrin and Uroporphyrin in Urine

Author:

Sobel Charles1,Cano Carlos1,Thiers Ralph E1

Affiliation:

1. Department of Research, Bio-Science Laboratories, Van Nuys, Calif. 91405

Abstract

Abstract Coproporphyrin and uroporphyrin are separated from urine and from each other by absorption onto, and selective elution from, an anion-exchange resin. The two compounds are separated by using solvents differing in both polarity and pH. The porphyrins are then quantitated with an Aminco-Bowman Spectrophotofluorometer equipped with an ellipsoidal mirror and a red-sensitive photomultiplier tube. Porphyrins can be determined in 1 ml of urine from a normal individual.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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