Affiliation:
1. Department of Public Health, Okayama University Medical School, 2-5-1 Shikata, Okayama, Japan
Abstract
Abstract
A simple method is described for the quantitative analysis of urinary δ-aminolevulinic acid. 2-Methyl3-carbethoxy-4-(3-propionic acid) pyrrole, produced by the condensation of δ-aminolevulinic acid with ethyl acetoacetate, is almost completely extracted from an aqueous solution with ethyl acetate without resorting to ion-exchange column chromatography. The pyrrole is determined colorimetrically by treating an aliquot of the extract with a modified Ehrlich’s reagent.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
101 articles.
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