Improved measurement of acetylsalicylic acid esterase in serum.

Author:

Sørensen S

Abstract

Abstract A fixed-time incubation method for measuring acetylsalicylic acid esterase was improved by using a higher concentration of the substrate, acetylsalicylic acid, and an activator, Ca2+. The enzymatic activity in serum was 25-fold that measured by earlier methods. Inhibition studies showed a pattern similar to that reported for cholinesterase. Use of cholinesterase inhibitor in sample tubes for determination of acetylsalicylic acid and salicylic acid is recommended.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

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