Enzymatic Determination of Glucose in Urine by Automation Following Rapid Removal of Inhibitors by Ion-Exchange Resin

Abstract

Abstract A commercial mixed-bed resin has been found effective for the rapid removal from urine of inhibitors of the glucose oxidase peroxidase system, thus allowing successful application of this specific method for glucose determination in urine specimens. The automated method of Hill and Kessler (10) for glucose determination was modified to make use of smaller quantities of the enzyme preparation and render the procedure less costly. The percentage inhibition produced by graded amounts of uric acid and ascorbic acid was determined. Both compounds were found to be completely removed by the resin treatment. Normal levels of uric acid in urine inhibit the enzyme reaction markedly, whereas only large therapeutic doses of ascorbic acid would produce sufficient levels of this compound in urine for an inhibitory action.