Urinary free catecholamines determined by liquid chromatography--fluorometry.

Author:

Anderson G M,Young J G,Jatlow P I,Cohen D J

Abstract

Abstract Free norepinephrine, epinephrine, and dopamine are determined in urine after purification on a small ion-exchange column and concentration on alumina. The catecholamines are separated by reversed-phase ion-pair chromatography and are quantitated by measuring their native fluorescence (lambda ex 285 nm, lambda em 305 nm). The internal standard is dihydroxybenzylamine. Within-day CVs for the compounds ranged from 1.6 to 5.6%; between-day CVs were 6.6 to 9.8%.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

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