Affiliation:
1. Toronto General Hospital, 101 College St., Toronto, Ontario, Canada M5G 1L7
Abstract
Abstract
We describe a modification of published methods for radioimmunoassay of plasma testosterone. This simpler method involves no chromatographic steps, and the necessary reagents, including tritiated testosterone and testosterone anti-serum, are commercially available. A digital computer is used for the calculation. Without difficulty, a technician can complete 100 assays in three working days. Mean testosterone concentrations (± 2 SD) in the plasmas of 21 normal men and 26 women with a normal menstrual cycle were 684 ± 300 and 45 ± 2O ng/dl, respectively. Within- and between-assay precision (coefficient of variation) were 5.2% (n = 29) and 6.7% (n = 26), respectively. We have assayed more than 1000 samples during the past year. We give data on the concentrations of testosterone in plasma of patients with various endocrine disorders.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
16 articles.
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