Affiliation:
1. Clinical Chemistry Laboratory, Hartford Hospital, Hartford, Conn. 06115
Abstract
Abstract
Findings with 9 self-indicating phenolic substrates for alkaline phosphatase assay by continuous spectrophotometric technic demonstrate that not only specific reaction conditions, e.g., buffer type, molarity, and pH, contribute significantly to the final observed "Sensitivity" attributed to any 1 substrate but also the following variables must be considered: (1) the enzyme's tissue and species source; (2) the molar absorptivity difference between the substrate and its dephosphorylated product; and (3) the molecular activity.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
16 articles.
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