Affiliation:
1. Department of Pathology, College of Medicine, University of Florida; and the Veterans Administration Hospital, Gainesville, Fla. 32601
Abstract
Abstract
Manual procedures are presented for determining immunoglobulins IgG, IgA, and IgM in human serum. The three immunoglobulins are quantitated, after reaction with specific antisera, by measuring light scattering by the antigen-antibody complexes. The procedure is standardized by use of pooled human serum with known concentrations of IgG, IgA, and IgM. Results with the present system correlated excellently with results of either radial immunodiffusion or an automated immunochemical procedure. Precision of duplicate determinations for the IgG, IgA, and IgM methods is 5.37, 1.30, and 5.36% (CV), respectively.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
67 articles.
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