Comparison of Four Methods for Measuring Uric Acid: Copper-Chelate, Phosphotungstate, Manual Uricase, and Automated Kinetic Uricase

Author:

Lum Gifford1,Gambino S Raymond1

Affiliation:

1. Clinical Chemistry Laboratory, Presbyterian Hospital, 630 West 168th St., New York, N. Y. 10032

Abstract

Abstract Glutathione, ascorbic acid and many commonly used antibiotics falsely elevate uric acid concentrations when measured with the Du Pont "aca," in which the copper-chelate method is used. L-Dopa interferes with both the copper-chelate method and the Technicon "SMA 12/60" phosphotungstate method. A new kinetic uricase method has been developed for use on the Du Pont aca. This kinetic uricase method has a coefficient of variation of 2.40% and gives values that are essentially identical to those obtained by a manual end-point uricase method.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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