Fractionation of Human Urine by Gel Chromatography

Author:

Burtis C A1,Goldstein Gerald1,Scott Charles D1

Affiliation:

1. Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830. Operated for the U.S. Atomic Energy Commission by the Union Carbide Corp

Abstract

Abstract High-resolution anion-exchange chromatography of human urine can resolve more than 150 constituents that absorb ultraviolet light. Prefractionation of urine on Sephadex G-10 helped us identify the constituents and simplified the anion-exchange chromatogram. The material associated with the 13 peaks obtained by gel chromatography was pooled and concentrated into six fractions, which were subsequently analyzed by high-resolution anion-exchange chromatography. Each of these fractions contained from 13 to 63 ultraviolet-absorbing constituents.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

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