Affiliation:
1. Children's Cancer Research Foundation and the Division of Laboratories and Research, The Children's Hospital Medical Center, Boston, Mass. 02115
Abstract
Abstract
Pure crystalline bilirubin was isolated from human, calf, hog, and dog gallbladder bile and compared with commercially-available materials. All may be purified by recrystallization from pyridine. The extinction coefficient in chloroform is 60,000-61,000 at 450-453 mµ.
Standardization of the analytical "azobilirubin" (van den Bergh) reaction may be based on aqueous alkaline solutions of bilirubin.
Paper chromatography of azobilirubins derived from various sources of bilirubin showed identical patterns.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
12 articles.
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