A Membrane Ultrafiltration Procedure for Determining Diffusible Calcium in Serum

Author:

Farese Genevieve1,Mager Milton1,Blatt William F1

Affiliation:

1. Biochemistry and Pharmacology Laboratory, U.S. Army Research Institute of Environmental Medicine, Natick, Mass. 01760, and Scientific Systems Division, Amicon Corporation, Lexington, Mass. 02173

Abstract

Abstract A rapid, simple procedure is described for separating diffusible from proteinbound serum calcium by centrifugation through high-flux ultrafiltration membranes. The mean normal value for ultrafilterable calcium was 57.1% (range 52 to 61%) of the total serum calcium concentration.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

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