Affiliation:
1. Seizure Clinic, Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Wash. 98195
Abstract
Abstract
We describe a modification of the MacGee method [Anal. Chem. 42, 421 ( 1970)] for rapid determination of phenytoin (diphenylhydantoin) in plasma by gas—liquid chromatography, in which an ethylating reagent is used instead of the more common methylating reagents. With this modification, several N-methylated antiepileptic drugs can be separated from their demethylated metabolites. Our results agree well with those of Butler and Waddell [Neurology 8, 106 ( 1958)], who showed that in patients receiving the N-methylated compounds mephenytoin and mephobarbital, the N-demethylated products, 5-ethyl-5-phenylhydantoin and phenobarbital are found in higher concentrations in plasma than are their respective parent drugs. The method is also useful for routine determinations of phenobarbital, primidone, and phenytoin in serum, cerebrospinal fluid, or saliva.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
14 articles.
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