Abstract
Abstract
We used automated and manual versions of two commonly used dye-binding methods for albumin to quantify albumin that had been modified by covalent addition of bilirubin. Dye-binding methods based on reaction with bromcresol purple underestimated the modified albumin by as much as 29%. Dye-binding methods based on reaction with bromcresol green were unaffected by the presence of covalently attached bilirubin. Neither method was affected by the presence of noncovalently bound bilirubin.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
27 articles.
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