Continuous-Flow Measurement of Lactate in Blood: A Technique Adapted for Use in the Emergency Laboratory-Reference-Cited by-同舟云学术

Continuous-Flow Measurement of Lactate in Blood: A Technique Adapted for Use in the Emergency Laboratory

Published:1975-01-01 Issue:1 Volume:21 Page:113-118
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Boycks Edward¹,Michaels Sybil¹,Weil Max Harry¹,Shubin Herbert¹,Marbach Edward P¹

Affiliation:

1. The Shock Research Unit and the Department of Medicine, University of Southern California School of Medicine, the Los Angeles County/USC Medical Center, and the Center for the Critically Ill, Hollywood Presbyterian Medical Center, Los Angeles, Calif. 90027

Abstract

Abstract This automated enzymatic method for analysis of lactate in blood, adapted from a manual method, is designed for use with an AutoAnalyzer. Start-up time is 5 min. After a time delay of 4 min, 99 samples may be analyzed per hour. Recovery averaged 100.7% (range, 99.6-101.8%). Reproducibility was within 1 SD (equivalent to 70 µmol/liter). Reagents are stable at room temperature for one month. The procedure has been in continuous use as an emergency test in support of emergency and critical care for three years, during which time we have done more than 10 000 determinations.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/21/1/113/32809103/clinchem0113.pdf

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