Specific Enzymatic Determination of Glucose in Blood Serum or Plasma by an Automatic Potentiometric Reaction-Rate Method

Author:

Malmstadt H V1,Pardue H L1

Affiliation:

1. Department of Chemistry and Chemical Engineering, University of Illinois, Urbana, Ill

Abstract

Abstract A new automatic potentiometric reaction-rate method has been applied to the specific enzymatic measurement of glucose in blood plasma or serum. A new filtering technic is described for removal of precipitated protein. The use of injection pipets to simplify and speed up the reagent-and sample-handling step is described. Glucose is determined in 0.02 ml. of serum or plasma with relative errors within 2%. The average measurement time is about 30 sec.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Cited by 14 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Methods for the determination of glucose in blood. Part 1;Review Journal of Chemistry;2015-01

2. Applications of Electrochemistry in the Design and Development of Medical Technologies and Devices;Modern Aspects of Electrochemistry;2013

3. The scientific legacy of Howard Vincent Malmstadt;Spectrochimica Acta Part B: Atomic Spectroscopy;2006-06

4. Glucose Oxidase as an Analytical Reagent;Critical Reviews in Analytical Chemistry;1995-09

5. Glucose oxidase: an ideal enzyme;Biosensors and Bioelectronics;1992-01

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