Quantitative Gas-Chromatographic Determination of Short-Chain Fatty Acids in Aqueous Samples-Reference-Cited by-同舟云学术

Quantitative Gas-Chromatographic Determination of Short-Chain Fatty Acids in Aqueous Samples

Published:1974-09-01 Issue:9 Volume:20 Page:1235-1237
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Collin Dennis P¹,McCormick Patrick G²,Schmitt Milton G³

Affiliation:

1. Department of Chemistry, Marquette University, Milwaukee, Wis. 53233.Department of Gastroenterology, Walter Reed Army Institute of Research, Washington, D. C. 20012

2. Department of Chemistry, Marquette University, Milwaukee, Wis. 53233.Pfizer, Inc., Quality Control Division, Brooklyn, N. Y. 11206

3. Department of Gastroenterology, Medical College of Wisconsin, Milwaukee, Wis. 53226. Present address: Rockford Clinic, 2300 North Rockton Ave., Rockford, Ill. 61101

Abstract

Abstract We report the use of SP-1200 (Supelco Inc.) for quantitative gas-chromatographic determination of short-chain fatty acids (C2-C5) in stool water. The ratio of peak areas for these acids to that for 2-methylvaleric acid (internal standard) is linear for each acid from 60 to 1800 mg/liter. Lactic acid, occasionally present in stool in abnormally high amounts, is not detectable as the free acid on SP-1200, but is determined as its propyl ester on diethyleneglycolsuccinate.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/20/9/1235/32821361/clinchem1235.pdf

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