Re-evaluation of EDTA-Chelated Biuret Reagent-Reference-Cited by-同舟云学术

Re-evaluation of EDTA-Chelated Biuret Reagent

Published:1974-10-01 Issue:10 Volume:20 Page:1362-1363
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Chromý V¹,Fischer J¹,Kulhánek V¹

Affiliation:

1. Research Institute of Pure Chemicals, Lachema, N. C., 621 33 Brno; and Institute of Medical Chemistry, School of Medicine, J. E. Purkyně University, Brno, Czechoslovakia

Abstract

Abstract We studied the optimal composition of EDTA-chelated biuret reagent for the determination of protein in serum. A reagent containing 18 mmol of EDTA, 15 mmol of Cu2+, and 1 mol of NaOH per liter exhibits a very low blank but about the same sensitivity as tartrate-chelated reagents. Maximum color intensity is attained within 25 min at room temperature, and adheres to Beer's law up to 120 g of protein per liter.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/20/10/1362/32825115/clinchem1362.pdf

Cited by 43 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Efforts toward optimization of aerobic biohydrogen reveal details of secondary regulation of biological nitrogen fixation by nitrogenous compounds in Azotobacter vinelandii;Applied Microbiology and Biotechnology;2018-09-24

2. Determination of proteins in blood. Part 3: Systematization of methods for the determination of protein compounds in blood;Review Journal of Chemistry;2017-07

3. The Role and the Place of High-Performance Liquid Chromatography for the Determination of Fermented Dairy Products;Soft Chemistry and Food Fermentation;2017

4. Determination of proteins in blood. Part 1: Determination of total protein and albumin;Review Journal of Chemistry;2017-01

5. 1,2-Propanediol Dehydration in Roseburia inulinivorans;Journal of Biological Chemistry;2016-07