Colorimetric Method for Hippuric Acid-Reference-Cited by-同舟云学术

Colorimetric Method for Hippuric Acid

Published:1963-02-01 Issue:1 Volume:9 Page:91-96
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Umberger Charles J¹,Fiorese Frank F¹

Affiliation:

1. Hospital for Special Surgery-Philip D. Wilson Research Foundation, affiliated with New York Hospital, Cornell University Medical Center

Abstract

Abstract Hippuric acid dissolved in pyridine producesa red-orange color upon the addition of benzenesulfonyl chloride. The color has a stable absorbance at 380 mµ. It is sensitive in microgram quantities of hippuric acid and follows the Beer-Lambert Law over a wide range of concentrations. The mechanism of the color reaction is described.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/9/1/91/32874113/clinchem0091.pdf

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