Use of High-Pressure Liquid Chromatography for Monitoring Nucleotide Concentration in Human Blood: A Preliminary Study with Stored Blood Cell Suspensions

Author:

Brown Phyllis R1,Parks R E1,Herod Jane1

Affiliation:

1. Section of Biochemical Pharmacology, Division of Biological and Medical Sciences, Brown University, Providence, R.I. 02912

Abstract

Abstract The development of high-pressure liquid chromatography has made available a new technique for monitoring nucleotide concentrations in biological materials sensitively, accurately, and rapidly. Because there appears to be a correlation between ATP concentrations and viability of stored blood, a preliminary study was carried out on blood cell suspensions to evaluate the technique for possible use in blood banks, clinical laboratories, and research on preservatives for blood.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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