Agar Gel Lipoprotein Electrophoresis: A Correlated Study with Ultracentrifugation

Author:

lammarino R M1,Humphrey M1,Antolik Paul1

Affiliation:

1. Department of Pathology, Clinical Chemistry Laboratory, and the Addison H. Gibson Laboratory of the University of Pittsburgh School of Medicine and Presbyterian-University Hospital, Pittsburgh, Pa 15213

Abstract

Abstract Interest in lipoprotein electrophoresis has been stimulated by a reappraisal of hyperlipoproteinemic syndromes and definition of five basic types according to Fredrickson (Types I, II, III, IV, V). The basic analytic reference for lipoprotein studies remains ultracentrifugal analysis. We have adapted an agar gel lipoprotein electrophoretic system employing a photometric scan and quantification of lipoprotein fractions based on post-electrophoresis Sudan Black B staining. This method of lipoprotein partitioning has been compared with lipoprotein analysis by ultracentrifugation. Statistically significant correlation between the methods is demonstrated.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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