A Saccharogenic Method for Estimating Electrophoretic and Chromatographic Distribution of Human Serum Amylase

Author:

Ujihira Ichiro1,Searcy Ronald L1,Berk J Edward1,Hayashi Shinichiro1

Affiliation:

1. Departments of Medicine and Pathology, California College of Medicine and Los Angeles County General Hospital (Unit 2), Los Angeles 33, Calif

Abstract

Abstract The dinitrosalicylic acid reaction for measuring reducing sugar has been evaluated and found suitable for determining the saccharogenic activity of human serum. The sensitivity and reproducibility of the modified dinitrosalicylic acid method permits quantitation of the electrophoretic and chromatographic distribution of human serum amylase. Such fractionations suggest that a majority, if not all, saccharogenic activity in normal and certain abnormal specimens is chromatographically separable from other serum protein and exhibits an electrophoretic mobility similar to γ-globulin.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

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