Affiliation:
1. Clinical Laboratory, Indiana University Medical Center, 1100 West Michigan St., Indianapolis, Ind. 46202
Abstract
Abstract
The reagent is modified as follows: Serum chromogens are destroyed by trisodium phosphate. The use of phosphotungstic acid eliminates the tungstate and sulfate anions, a frequent cause of turbidity. Triethanolamine, added to the carbonate—urea mixture, provides unusual stability of the color through its buffering action. Only 0.2 ml of serum is required. The method is highly reproducible and accurate; analytical results agree closely with those obtained by the uricase or cyanide—urea method.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
14 articles.
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