Multiple labeling and time-resolvable fluorophores

Author:

Diamandis E P1

Affiliation:

1. Department of Clinical Biochemistry, Toronto Western Hospital, Ontario, Canada

Abstract

Abstract A new time-resolved fluorescence immunoassay system involving use of the europium chelate of 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid as label is reviewed. This stable chelate by itself is not very fluorescent but, used in multiple labeling strategies, improves the achievable detection limits. By using multiple labeling, streptavidin tailing, and Eu3+ activation, one can create a very stable, easy-to-use reagent that is suitable for devising highly sensitive immunoassays and other biotechnological assays. This reagent, a streptavidin-based macromolecular complex, is able to detect approximately 300,000 molecules (approximately 0.5 amol) of alpha-fetoprotein in a model noncompetitive immunoassay.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Cited by 35 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. A Novel Europium Chelate Coated Nanosphere for Time-Resolved Fluorescence Immunoassay;PLOS ONE;2015-06-09

2. Simple no-chromatography procedure for amine-reactive Eu3+ luminescent chelates optimal for bioconjugation;Journal of Photochemistry and Photobiology A: Chemistry;2013-03

3. Highly bright avidin-based affinity probes carrying multiple lanthanide chelates;Journal of Photochemistry and Photobiology B: Biology;2012-11

4. Advancing Laboratory Medicine through Innovation: A Tale of Six Inventors;Clinical Chemistry;2012-03-01

5. Principles of Immunochemical Techniques;Tietz Textbook of Clinical Chemistry and Molecular Diagnostics;2012

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