Affiliation:
1. Pathology Department, South Side Hospital, S. 20th and Jane Sts., Pittsburgh, Pa. 15203.
Abstract
Abstract
The use of sodium thymolphthalein monophosphate in measuring alkaline phosphatase activity of serum is described. Thymolphthalein liberated in the reaction is conveniently measured by raising the pH of the medium, which produces a color and also stops the hydrolysis. The effects of pH, kind and molarity of buffer, temperature, and enzyme and magnesium con-centrations were studied. Results by this procedure correlate well with those obtained by a standard procedure in which liberated p-nitrophenol is de-termined. Advantages of the present method include excellent linearity, high precision, short incubation, and reagent stability.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry