Affiliation:
1. Kitano Hospital, Tazuke Kofukai Medical Research Institute, Kita-ku, Osaka City, Japan
Abstract
Abstract
A simplified method for the determination of pepsinogen in blood and urine has been developed using serum protein as substrate. As crystalline pepsin digests human serum protein optimally in the region of pH 2, and blood exhibits such proteolytic activity on its own serum protein in this region, the determination of pepsinogen in blood is performed at this pH. The proteolytic activity of serum or plasma on its own (autologous) serum protein is inactivated by alkalinization. The blood of gastrectomized patients showed extremely low proteolytic activity in the region of pH 2 by the present method, indicating an absence of pepsinogen. The present method has greater specificity and accuracy than the method employing either hemoglobin or casein as substrate.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
18 articles.
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