Using eDNA to play whack-a-mole with invasive species in green yard waste

Author:

Neven Lisa G1ORCID,Walker William B1ORCID,Gowton Chelsea2,Carrillo Juli2

Affiliation:

1. Temperate Tree Fruit and Vegetable Research Unit, USDA-ARS , 5230 Konnowac Pass Road, Wapato, WA 98951 , USA

2. Centre for Sustainable Food Systems, Biodiversity Research Centre, University of British Columbia , 2357 Main Mall, Vancouver, BC V6T 1Z4 , Canada

Abstract

Abstract As large cities begin to overrun their landfill capacities, they begin to look for alternative locations to handle the waste stream. Seeing an opportunity to bring in revenue, rural communities offer to handle municipal waste in their landfills. However, many rural communities are also places of agricultural production, which are vulnerable to attacks by invasive insect species, which could be present in green yard waste, the component of municipal waste most likely to contain agriculturally harmful insect species. We used environmental DNA (eDNA) to determine whether green yard waste could be a pathway for invasive insect species to enter and establish in the landfill-receiving agricultural community. We identified several target species that could be in green yard waste coming from Vancouver, BC, Canada, to Central Washington State, USA. We sampled green yard waste from 3 sites every 2 weeks from June to October in 2019 and 2020. DNA was extracted from the nearly 400 samples and subjected to amplification with COI barcoding primers followed by sequencing to identify target insects in the samples. Sequence analyses identified 3 species from the target list: 2 species that are pests of deciduous tree fruits and a generalist root-feeding crop pest. This eDNA technique was useful in identifying potential invasive species in green yard waste and may prove to be an important tool informing policy on the movement of biological material across borders and stemming the spread of invasive species.

Funder

Washington State Department of Agriculture

Publisher

Oxford University Press (OUP)

Reference43 articles.

1. Terrestrial eDNA survey outperforms conventional approach for detecting an invasive pest insect within an agricultural ecosystem;Allen;Environ DNA,2021

2. Chapter 5 tortricids in pome and stone fruits. 5.1.1 Codling moth occurrence, host race formation and damage;Barnes,1991

3. Incorporating sustainable and technological approaches in pest management of invasive arthropod species;Bernaola;Ann Entomol Soc Am,2021

4. Massive yet grossly underestimated global costs of invasive insects;Bradshaw;Nat Commun,2016

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3