Affiliation:
1. Interdisciplinary Plant Group (IPG), Division of Biochemistry, University of Missouri–Columbia, Columbia, Missouri 65211
2. Division of Plant Sciences, University of Missouri–Columbia, Columbia, Missouri 65211
3. Department of Biochemistry, University of Wisconsin–Madison, Madison, Wisconsin 53706
Abstract
Abstract
Ligand-induced endocytosis of the immune receptor FLAGELLIN SENSING2 (FLS2) is critical for maintaining its proper abundance in the plasma membrane (PM) to initiate and subsequently down regulate cellular immune responses to bacterial flagellin or flg22-peptide. The molecular components governing PM abundance of FLS2, however, remain mostly unknown. Here, we identified Arabidopsis (Arabidopsis thaliana) DYNAMIN-RELATED PROTEIN1A (DRP1A), a member of a plant-specific family of large dynamin GTPases, as a critical contributor to ligand-induced endocytosis of FLS2 and its physiological roles in flg22-signaling and immunity against Pseudomonas syringae pv. tomato DC3000 bacteria in leaves. Notably, drp1a single mutants displayed similar flg22-defects as those previously reported for mutants in another dynamin-related protein, DRP2B, that was previously shown to colocalize with DRP1A. Our study also uncovered synergistic roles of DRP1A and DRP2B in plant growth and development as drp1a drp2b double mutants exhibited severely stunted roots and cotyledons, as well as defective cell shape, cytokinesis, and seedling lethality. Furthermore, drp1a drp2b double mutants hyperaccumulated FLS2 in the PM prior to flg22-treatment and exhibited a block in ligand-induced endocytosis of FLS2, indicating combinatorial roles for DRP1A and DRP1B in governing PM abundance of FLS2. However, the increased steady-state PM accumulation of FLS2 in drp1a drp2b double mutants did not result in increased flg22 responses. We propose that DRP1A and DRP2B are important for the regulation of PM-associated levels of FLS2 necessary to attain signaling competency to initiate distinct flg22 responses, potentially through modulating the lipid environment in defined PM domains.
Funder
National Science Foundation
National Institute of Health
Initiative Maximizing Student Diversity
University of Missouri
Diane P. and Robert E. Sharp Fund Fellowship
Daniel F. Millikan Graduate Fellowship
MU-College of Agriculture, Natural Food and Resources-Undergraduate Research Internship
Dudley Alexander Undergraduate Research On-Campus Internships Gift Fund
MU-Discovery Fellowship
MU-Life Sciences Undergraduate Research Opportunity Fellowship
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
Cited by
14 articles.
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