Transcription factor BZR2 activates chitinase Cht20.2 transcription to confer resistance to wheat stripe rust

Author:

Bai Xingxuan1,Zhan Gangming1,Tian Shuxin1,Peng Huan1,Cui Xiaoyu2,Islam Md Ashraful1ORCID,Goher Farhan1,Ma Youzhi2,Kang Zhensheng1,Xu Zhao-Shi2ORCID,Guo Jun1ORCID

Affiliation:

1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, P.R. China

2. Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/ Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, P.R. China

Abstract

Abstract The brassinosteroid pathway promotes a variety of physiological processes in plants and the brassinosteroid insensitive1-ethylmethane sulfonate suppressor (BES)/brassinazole-resistant (BZR) functions as one of its key regulators. We previously showed that the BES/BZR-type transcription factor TaBZR2 mediates the drought stress response in wheat (Triticum aestivum) by directly upregulating the transcriptional activity of glutathione S-transferase 1. However, the function of TaBZR2 in plants under biotic stresses is unknown. In this study, we found that transcript levels of TaBZR2 were upregulated in response to inoculation with wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, Pst) and treatment with flg22 or an elicitor-like protein of Pst, Pst322. Wheat lines overexpressing TaBZR2 conferred increased resistance, whereas TaBZR2-RNAi lines exhibited decreased resistance to multiple races of Pst. TaBZR2 targeted the promoter of the chitinase gene TaCht20.2, activating its transcription. Knockdown of TaCht20.2 in wheat resulted in enhanced susceptibility to Pst, indicating the positive role of TaCht20.2 in wheat resistance. Upon Pst infection in vivo, the overexpression of TaBZR2 increased total chitinase activity, whereas RNAi-mediated silencing of TaBZR2 reduced total chitinase activity. Taken together, our results suggest that TaBZR2 confers broad-spectrum resistance to the stripe rust fungus by increasing total chitinase activity in wheat.

Funder

National Natural Science Foundation of China

Key Research and Development Program of Shaanxi

National Key R&D Program of China

Natural Science Basic Research Program of Shaanxi

111 Project from the Ministry of Education of China

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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