The NAC transcription factor ANAC017 regulates aluminum tolerance by regulating the cell wall-modifying genes

Author:

Tao Ye12,Wan Jiang Xue3,Liu Yu Song12,Yang Xiao Zheng12,Shen Ren Fang12ORCID,Zhu Xiao Fang12ORCID

Affiliation:

1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science , Nanjing 210008, China

2. University of Chinese Academy of Sciences , Beijing 100049, China

3. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China (in preparation), Rice Research Institute, Sichuan Agricultural University , Chengdu 611130, China

Abstract

Abstract Aluminum (Al) toxicity is one of the key factors limiting crop production in acid soils; however, little is known about its transcriptional regulation in plants. In this study, we characterized the role of a NAM, ATAF1/2, and cup-shaped cotyledon 2 (NAC) transcription factors (TFs), ANAC017, in the regulation of Al tolerance in Arabidopsis (Arabidopsis thaliana). ANAC017 was localized in the nucleus and exhibited constitutive expression in the root, stem, leaf, flower, and silique, although its expression and protein accumulation were repressed by Al stress. Loss of function of ANAC017 enhanced Al tolerance when compared with wild-type Col-0 and was accompanied by lower root and root cell wall Al content. Furthermore, both hemicellulose and xyloglucan content decreased in the anac017 mutants, indicating the possible interaction between ANAC017 and xyloglucan endotransglucosylase/hydrolase (XTH). Interestingly, the expression of XTH31, which is responsible for xyloglucan modification, was downregulated in the anac017 mutants regardless of Al supply, supporting the possible interaction between ANAC017 and XTH31. Yeast one-hybrid, dual-luciferase reporter assay, and chromatin immunoprecipitation-quantitative PCR analysis revealed that ANAC017 positively regulated the expression of XTH31 through directly binding to the XTH31 promoter region, and overexpression of XTH31 in the anac017 mutant background rescued its Al-tolerance phenotype. In conclusion, we identified that the tTF ANAC017 acts upstream of XTH31 to regulate Al tolerance in Arabidopsis.

Funder

Foundation for Distinguished Young Scholars of Jiangsu Province

National Natural Science Foundation of China

Youth Innovation Promotion Association of Chinese Academy of Sciences

National Science Foundation of China

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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