Electron microscopy for imaging organelles in plants and algae

Author:

Weiner Ethan12ORCID,Pinskey Justine M3,Nicastro Daniela3ORCID,Otegui Marisa S12ORCID

Affiliation:

1. Department of Botany, University of Wisconsin, Madison 53706, Wisconsin

2. Center for Quantitative Cell Imaging, University of Wisconsin, Madison 53706, Wisconsin

3. Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas 75390, Texas

Abstract

Abstract Recent developments in both instrumentation and image analysis algorithms have allowed three-dimensional electron microscopy (3D-EM) to increase automated image collections through large tissue volumes using serial block-face scanning EM (SEM) and to achieve near-atomic resolution of macromolecular complexes using cryo-electron tomography (cryo-ET) and sub-tomogram averaging. In this review, we discuss applications of cryo-ET to cell biology research on plant and algal systems and the special opportunities they offer for understanding the organization of eukaryotic organelles with unprecedently resolution. However, one of the most challenging aspects for cryo-ET is sample preparation, especially for multicellular organisms. We also discuss correlative light and electron microscopy (CLEM) approaches that have been developed for ET at both room and cryogenic temperatures.

Funder

Otegui Laboratory

National Science Foundation

National Institute of Health

The Cryo-EM work in the Nicastro Laboratory is supported by grants from the National Institutes of Health

Cancer Prevention and Research Institute of Texas

UT Southwestern cryo-EM Facility

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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