The transcription factor ORA59 exhibits dual DNA binding specificity that differentially regulates ethylene- and jasmonic acid-induced genes in plant immunity

Author:

Yang Young Nam1,Kim Youngsung1,Kim Hyeri1,Kim Su Jin1,Cho Kwang-Moon2,Kim Yerin3ORCID,Lee Dong Sook1,Lee Myoung-Hoon1,Kim Soo Young4ORCID,Hong Jong Chan5ORCID,Kwon Sun Jae2,Choi Jungmin3,Park Ohkmae K1ORCID

Affiliation:

1. Department of Life Sciences, Korea University, Seoul 02841, Korea

2. Molecular Diagnosis Division, AccuGene, Incheon 22006, Korea

3. Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Korea

4. Department of Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea

5. Division of Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Korea

Abstract

Abstract Jasmonic acid (JA) and ethylene (ET) signaling modulate plant defense against necrotrophic pathogens in a synergistic and interdependent manner, while JA and ET also have independent roles in certain processes, e.g. in responses to wounding and flooding, respectively. These hormone pathways lead to transcriptional reprogramming, which is a major part of plant immunity and requires the roles of transcription factors. ET response factors are responsible for the transcriptional regulation of JA/ET-responsive defense genes, of which ORA59 functions as a key regulator of this process and has been implicated in the JA-ET crosstalk. We previously demonstrated that Arabidopsis (Arabidopsis thaliana) GDSL LIPASE 1 (GLIP1) depends on ET for gene expression and pathogen resistance. Here, promoter analysis of GLIP1 revealed ERELEE4 as the critical cis-element for ET-responsive GLIP1 expression. In a yeast one-hybrid screening, ORA59 was isolated as a specific transcription factor that binds to the ERELEE4 element, in addition to the well-characterized GCC box. We found that ORA59 regulates JA/ET-responsive genes through direct binding to these elements in gene promoters. Notably, ORA59 exhibited a differential preference for GCC box and ERELEE4, depending on whether ORA59 activation is achieved by JA and ET, respectively. JA and ET induced ORA59 phosphorylation, which was required for both activity and specificity of ORA59. Furthermore, RNA-seq and virus-induced gene silencing analyses led to the identification of ORA59 target genes of distinct functional categories in JA and ET pathways. Our results provide insights into how ORA59 can generate specific patterns of gene expression dynamics through JA and ET hormone pathways.

Funder

Korea University grant and National Research Foundation of Korea

Korean government

NRF

Korean government (MSIP

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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