Soybean ZINC FINGER PROTEIN03 targets two SUPEROXIDE DISMUTASE1s and confers resistance to Phytophthora sojae

Author:

Li Wei1ORCID,Zheng Xiang1,Cheng Rong1,Zhong Chanjuan1,Zhao Jie2,Liu Tyler H3,Yi Tuyong1,Zhu Zhendong4ORCID,Xu Jieting5ORCID,Meksem Khalid6ORCID,Dai Liangying1ORCID,Liu Shiming12ORCID

Affiliation:

1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , P. R. China

2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193 , P. R. China

3. College of Letters and Science, University of Wisconsin , Madison, WI 53706 , USA

4. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing 100081 , P. R. China

5. Wimi Biotechnology Co., Ltd , Changzhou 213000 , P. R. China

6. Department of Plant, Soil and Agricultural Systems, Southern Illinois University , Carbondale, IL 62901 , USA

Abstract

Abstract Phytophthora sojae causes Phytophthora root and stem rot disease of soybean (Glycine max), leading to huge annual yield loss worldwide, but resistance to Phytophthora sojae (Rps) genes remains elusive. Soybean cultivar “Yudou 29” is resistant to P. sojae strain PsMC1, and this study aimed to clone, identify, and characterize the Rps gene in Yudou 29 (RpsYD29) and clarify its functional mechanism. We map-based cloned RpsYD29 (ZINC FINGER PROTEIN03, GmZFP03) using the families of a cross between Yudou 29 and a P. sojae-susceptible soybean cultivar “Jikedou 2”. P. sojae resistance of GmZFP03 was functionally validated by stable soybean genetic transformation and allele-phenotype association analysis. GmZFP03 was identified as a C2H2-type zinc finger protein transcription factor, showing 4 amino acid residue polymorphisms (V79F, G122-, G123-, and D125V) and remarkably different expression patterns between resistant and susceptible soybeans. Notably boosted activity and gene expression of superoxide dismutase (SOD) in resistant-type GmZFP03-expressed transgenic soybean, substantial enhancement of P. sojae resistance of wild-type soybean by exogenous SOD treatment, and GmZFP03 binding to and activation of 2 SOD1 (Glyma.03g242900 and Glyma.19g240400) promoters demonstrated the involvement of SOD1s in GmZFP03-mediated resistance to P. sojae strain PsMC1. Thus, this study cloned the soybean P. sojae-resistant GmZFP03, the product of which specifically targets 2 SOD1 promoters. GmZFP03 can be directly used for precise P. sojae-resistance soybean breeding.

Funder

Shennong Scholar Program of Hunan Agricultural University

National Natural Science Foundation of China

Crop Germplasm Conservation, and Utilization Program from the Ministry of Agriculture of China

Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences

the Science and Technology Project of Hunan Province

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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