Transcription factor bZIP52 modulates Arabidopsis seed oil biosynthesis through interaction with WRINKLED1

Author:

Yang Yuzhou1ORCID,Kong Que1ORCID,Tee Wan Ting1ORCID,Li Yuqing23ORCID,Low Pui Man1ORCID,Patra Barunava4ORCID,Guo Liang23ORCID,Yuan Ling4ORCID,Ma Wei1ORCID

Affiliation:

1. School of Biological Sciences, Nanyang Technological University , Singapore 637551 , Singapore

2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University , Wuhan 430070 , China

3. Hubei Hongshan Laboratory , Wuhan 430070 , China

4. Department of Plant and Soil Sciences, Kentucky Tobacco Research and Development Center, University of Kentucky , Lexington, KY 40546 , USA

Abstract

Abstract Transcriptional regulation mediated by combinatorial interaction of transcription factors (TFs) is a key molecular mechanism modulating plant development and metabolism. Basic leucine zipper (bZIP) TFs play important roles in various plant developmental and physiological processes. However, their involvement in fatty acid biosynthesis is largely unknown. Arabidopsis (Arabidopsis thaliana) WRINKLED1 (WRI1) is a pivotal TF in regulation of plant oil biosynthesis and interacts with other positive and negative regulators. In this study, we identified two bZIP TFs, bZIP21 and bZIP52, as interacting partners of AtWRI1 by yeast-two-hybrid (Y2H)–based screening of an Arabidopsis TF library. We found that coexpression of bZIP52, but not bZIP21, with AtWRI1 reduced AtWRI1-mediated oil biosynthesis in Nicotiana benthamiana leaves. The AtWRI1–bZIP52 interaction was further verified by Y2H, in vitro pull-down, and bimolecular fluorescence complementation assays. Transgenic Arabidopsis plants overexpressing bZIP52 showed reduced seed oil accumulation, while the CRISPR/Cas9-edited bzip52 knockout mutant exhibited increased seed oil accumulation. Further analysis revealed that bZIP52 represses the transcriptional activity of AtWRI1 on the fatty acid biosynthetic gene promoters. Together, our findings suggest that bZIP52 represses fatty acid biosynthesis genes through interaction with AtWRI1, resulting in a reduction of oil production. Our work reports a previously uncharacterized regulatory mechanism that enables fine-tuning of seed oil biosynthesis.

Funder

Ministry of Education

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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