A small chromosomal inversion mediated by MITE transposons confers cleistogamy in Brassica napus
Author:
Wan Shubei1ORCID,
Yang Mao1ORCID,
Ni Fei1ORCID,
Chen Wenjing1ORCID,
Wang Yangming1,
Chu Pu12ORCID,
Guan Rongzhan12ORCID
Affiliation:
1. State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University , Nanjing 210095, China
2. Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University , Nanjing 210095, China
Abstract
Abstract
Cleistogamy, self-pollination within closed flowers, can help maintain seed purity, accelerate breeding speed, and aid in the development of ornamental flowers. However, the mechanism underlying petal closing/opening behavior remains elusive. Here, we found that a Brassica napus petal closing/opening behavior was inherited in a Mendelian manner. Fine mapping and positional cloning experiments revealed that the Mendelian factor originated from a short (29.8 kb) inversion mediated by BnDTH9 miniature inverted-repeat transposable elements (MITEs) on chromosome C03. This inversion led to tissue-specific gene promoter exchange between BnaC03.FBA (BnaC03G0156800ZS encoding an F-Box-associated domain-containing protein) and BnaC03.EFO1 (BnaC03G0157400ZS encoding an EARLY FLOWERING BY OVEREXPRESSION 1 protein) positioned near the respective inversion breakpoints. Our genetic transformation work demonstrated that the cleistogamy originated from high tissue-specific expression of the BnaC03.FBA gene caused by promoter changes due to the MITE-mediated inversion. BnaC03.FBA is involved in the formation of an SCF (Skp1–Cullin–F-box) complex, which participates in ubiquitin-mediated protein targeting for degradation through the ubiquitin 26S-proteasome system. Our results shed light on a molecular model of petal-closing behavior.
Funder
National Natural Science Foundation of China
National Science and Technology Major Project of the Ministry of Science and Technology of China
Fundamental Research Funds for the Central Universities
Cyrus Tang Seed Innovation Center, Nanjing Agricultural University
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
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