HISTONE DEACETYLASE 15 and MOS4-associated complex subunits 3A/3B coregulate intron retention of ABA-responsive genes

Author:

Tu Yi-Tsung1ORCID,Chen Chia-Yang1,Huang Yi-Sui1ORCID,Chang Chung-Han1ORCID,Yen Ming-Ren2ORCID,Hsieh Jo-Wei Allison23ORCID,Chen Pao-Yang23ORCID,Wu Keqiang1ORCID

Affiliation:

1. Institute of Plant Biology, National Taiwan University , Taipei 10617, Taiwan

2. Institute of Plant and Microbial Biology, Academia Sinica , Taipei 11529, Taiwan

3. Genome and Systems Biology Degree Program, Academia Sinica and National Taiwan University , Taipei 10617, Taiwan

Abstract

Abstract Histone deacetylases (HDAs) play an important role in transcriptional regulation of multiple biological processes. In this study, we investigated the function of HDA15 in abscisic acid (ABA) responses. We used immunopurification coupled with mass spectrometry-based proteomics to identify proteins interacting with HDA15 in Arabidopsis (Arabidopsis thaliana). HDA15 interacted with the core subunits of the MOS4-associated complex (MAC), MAC3A and MAC3B, with interaction between HDA15 and MAC3B enhanced by ABA. hda15 and mac3a/mac3b mutants were ABA-insensitive during seed germination and hyposensitive to salinity. RNA sequencing analysis demonstrated that HDA15 and MAC3A/MAC3B co-regulate ABA-responsive intron retention (IR). Furthermore, HDA15 reduced the histone acetylation level of genomic regions near ABA-responsive IR sites and the association of MAC3B with ABA-responsive pre-mRNA was dependent on HDA15. Our results indicate that HDA15 is involved in ABA responses by interacting with MAC3A/MAC3B to mediate splicing of introns.

Funder

Ministry of Science and Technology of the Republic of China

National Taiwan University

NTU-Academia Sinica

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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