Liquid Chromatographic Method for the Quantification of Zearalenone in Baby Food and Animal Feed: Interlaboratory Study
Author:
Arranz Isabel1, Mischke Carsten1, Stroka Joerg1, Sizoo Eric2, van Egmond Hans2, Neugebauer Michael3, Biselli S, Bonzaaijer G, Breyl I, Brodacz W, Bujara I, Burdaspal P, Cea J, Chan D, Danier J, Debreczeni L, De Girolamo A, De Rechter P, De Saeger S, Dittmar F, Esteves M E, Frohmuth G, González P, Griffin J, Hackenberg R, Hanschmann G, Iversen A, Jaus A, Just P, Lauber U, Legarda T, Li Feng-Qin, Meister U, Michelet J Y, Michels K, Mrkvilova M, Nordkvist E, Nuotio K, Özkaya Ş, Patel S, Petrová J, Pittet; A, Raditschnig A, Reutter M, Ritscher M, Schenerr H, Slezarova A, Sona T, van Osenbruggen T, Van Peteghem C, Vojsová Y, Wilson P, Wisniewska-Dmytrow H, Woese K,
Affiliation:
1. Institute for Reference Materials and Measurements, European CommissionJoint Research Center, Food Safety and Quality Unit, Retieseweg 111, B-2440 Geel, Belgium 2. National Institute for Public Health and the Environment, Laboratory for Food and Residue Analysis, PO Box 1, 3720 Bilthoven, The Netherlands 3. University of Bonn, Pharmaceutical Chemistry, An der Immenburg 4, D-53121 Bonn, Germany
Abstract
Abstract
An interlaboratory trial for determination of zearalenone (ZON) in baby food and animal feed was conducted. The study involved 39 participants in 16 European Union member states, as well as Turkey, Uruguay, and China, representing a cross-section of industry, and official food control and research institutes. The method is based on immunoaffinity column cleanup followed by high-performance liquid chromatography using fluorimetry (HPLC-Fl). The test portion of the sample is extracted with methanolwater (75 + 25, v/v). The sample extract is filtered, diluted, and passed over an immunoaffinity column. ZON is eluted with methanol. The separation and determination of ZON is performed by reversed-phase HPLC-Fl with an excitation wavelength of 274 nm and an emission wavelength of 446 nm. Test portions of the samples were spiked at levels of 20 and 30 g/kg ZON in baby food and at levels of 100 and 150 g/kg ZON in animal feed. Mean recoveries from each participant ranged from 78 to 119 with an average value of 92 for baby food and from 51 to 122 with an average value of 74 for animal feed. Based on results for spiked samples (blind duplicates at 2 levels), as well as naturally contaminated samples (blind duplicates at 3 levels), the relative standard deviation for repeatability (RSDr) in baby food ranged from 2.8 to 9.0. For animal feed, this value ranged from 5.7 to 9.5. The relative standard deviation for reproducibility (RSDR) in baby food ranged from 8.2 to 13.3, and for animal feed this value ranged from 15.5 to 21.4. The Horwitz ratio (HorRat) in baby food ranged from 0.3 to 0.4, and for animal feed this value ranged from 0.6 to 0.9. The method showed acceptable within-and between-laboratory precision for each matrix, as required by European legislation.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
12 articles.
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