Determination of Patulin in Apple Juice: Comparative Evaluation of Four Analytical Methods

Author:

Katerere David R1,Stockenström Sonja1,Balducci Gabriele2,Shephard Gordon S3

Affiliation:

1. Programme on Mycotoxins and Experimental Carcinogenesis (PROMEC), Medical Research Council, PO Box 19070, Tygerberg 7505, South Africa

2. Universit degli Studi di Bologna, Corso di Laurea in Scienze e Tecnologie Alimentari via Ravennate, Cesena, Italy

3. PROMEC, Medical Research Council, PO Box 19070, Tygerberg 7505, South Africa

Abstract

Abstract The performance of 4 purification methods for the analysis of patulin in apple juice was evaluated by high-performance liquid chromatography (HPLC). Samples were spiked with patulin at 10, 20, 50, 100, and 150 ppb (ng/mL) and extracted by one of 4 methods (3 solid-phase extraction and one liquidliquid extraction), and then analyzed by HPLCUV under the same isocratic conditions. The methods were validated for recovery, linearity, and precision at high and low concentrations. Recoveries were all >70% for spiking range 10-150 ppb. The relative standard deviation for repeatability was found to meet European Union Directive requirements. In addition, all the methods showed baseline separation from hydroxymethylfurfural.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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