Chemical Fingerprinting of Hoodia Species and Related Genera: Chemical Analysis of Oxypregnane Glycosides Using High-Performance Liquid Chromatography with UV Detection in Hoodia gordonii-Reference-Cited by-同舟云学术

Chemical Fingerprinting of Hoodia Species and Related Genera: Chemical Analysis of Oxypregnane Glycosides Using High-Performance Liquid Chromatography with UV Detection in Hoodia gordonii

Published:2007-11-01 Issue:6 Volume:90 Page:1526-1531
ISSN:1060-3271
Container-title:Journal of AOAC INTERNATIONAL
language:en
Short-container-title:

Author:

Avula Bharathi¹,Wang Yan-Hong¹,Pawar Rahul S¹,Shukla Yatin J²,Khan Ikhlas A³

Affiliation:

1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677

2. The University of Mississippi, School of Pharmacy, Department of Pharmacognosy, University, MS 38677

3. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, Department of Pharmacognosy, The University of Mississippi, University, MS 38677

Abstract

Abstract Hoodia gordonii, family Asclepiadaceae, is a succulent plant and is traditionally used in southern Africa for its appetite-suppressant properties. A high-performance liquid chromatographic (HPLC) method with UV detection for analysis of 11 oxypregnane glycosides from H. gordonii has been developed. The simultaneous analysis of 11 oxypregnane glycosides was achieved with a Phenomenex (Torrance, CA) reversed-phase C18 column using gradient mobile phase of water and acetonitrile, both containing 0.025 trifluoroacetic acid. The developed method was applied to the identification of oxypregnane glycosides in 3 different species of Hoodia and 23 related genera. The HPLC profiles of various plant samples were compared for the presence of oxypregnane glycosides.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

Link

http://academic.oup.com/jaoac/article-pdf/90/6/1526/32419552/jaoac1526.pdf

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